The boys from Terry Brown's AFC Wimbledon were away to Kenilworth Road in Luton, Bedfordshire to take on the Hatters of Richard Money's Luton Town F.C.
The Dons were coming off a comprehensive 4-0 shutout victory over Bath City at Kingsmeadow's Cherry Red Records Stadium.
“The first half was probably the best we have played all season," Brown said on the win. "I thought credit should go to Bath City who came to Kingsmeadow to play football and not pull everyone back behind the ball.
"We didn’t reassert ourselves after the half time interval and started off very slowly, which gave our visitors the chance to get back into the game.
“In the end, the four-nil result did not flatter us and I was particularly pleased that two goals were scored from set pieces, which is a benefit from being full time and having the opportunity to practice.
"All the teams at the top have again won and we now face in our next two games two of the biggest challenges. I will know a lot more about our team and our chances for the season ahead after those two games.”
The match was televised on Premier Sports.
"Yesterday's training went without a hitch, although a couple of players were unable to train and will have fitness tests this morning," Brown said during training. "Normally I'd say who it is, but I don't particularly want to tell Luton, so I am very sorry but I will have to leave you all in suspense about their identities.
"Nonetheless, we go into tonight's game in a confident mood after a very good display against Bath on Saturday. Having looked at Luton's report against Cambridge in their 2-0 win and we should therefore look forward to an open and exciting encounter tonight.
"For several of our players, it will be one of the biggest games they have played in to date and it will be interesting to see how they rise to the occasion. The position we find ourselves in league-wise will hopefully allow us to feel confident enough to express ourselves and play our normal game as we have absolutely nothing to fear with regards the result."
Unfortunately for the Dons, it wasn't the best of performances. Goals by George Pilkington in the 23rd minute, Zdenek Kroca in the 37th minute, and Matther Barnes-Horner in the 49th minute were enough to deal Luton Town the Dons' second defeat of the season, a 3-0 shutout loss.
A victory by Crawley Town this week will set up a pivotal showdown to determine first place in the Football Conference National. The Dons need to shake off what has been their worst outing of the young season to date in order get their form back.
Kickoff at Kingsmeadow is scheduled for Thursday, Sept. 23, at 3:45 p.m. ET.