The Bianconeri will look to field their best XI, with Antonio Conte's signature 3-5-2 formation giving them plenty of cover and bite in hostile territory.
Despite this, they'll encounter a rabid Bayern Munich side that has dominated the Bundesliga and is widely regarded as one of the favorites to win the Champions League trophy.
The legendary Italian goalkeeper has been lauded as one of the finest between the pipes that we've ever seen.
Noted for his stellar performance in the 2006 World Cup, which helped his nation win it, Gigi has not lost his form as he's been fantastic when called upon.
Buffon was a key catalyst in the 49-match undefeated streak Juventus had earlier in the season, and Bayern Munich will need a perfectly placed strike to get by him.
Despite being portrayed as the weak link in the defensive line, many who see Leonardo Bonucci play weekly know where his true skill set lies.
He's one of the finest ball-playing defenders in Europe, and he often starts the attack from the back and pushes the ball upfield with lobs that would arouse the most accurate of deep-lying playmakers.
Bonucci works perfect in a three-man defense, where the other two can do the dirty work for him while he plies his trade with finesse.
Juventus still can't believe their luck after picking up Andrea Barzagli from the bargain bin of Wolfsburg.
Ever since his arrival two seasons ago, Andrea has become the best central defender in Italy. A remarkable rise from a player who was virtually forgotten about by the Italians and their national team when he left Palermo to go to Wolfsburg
An elegant defender who rarely makes mistakes, it'll take a great effort for the Bayern strikers to get their way past Barzagli. He reminds plenty of Alessandro Nesta with his surgical timing on interceptions.
Providing the toughness and grit in the defense, Giorgio Chiellini is a natural leader and an enforcer at the back.
Despite now being billed as a central defender, he started his career as a full-back and is capable of going forward effectively. Chiellini is also an intimidating aerial presence and fantastic at headers.
The fiery Swiss right wing-back has one of the most important roles in the Juventus formation. He has to track back and defend while also venturing forward and providing much needed width.
Stephan Lichtsteiner has an impressive work rate and is a fine crosser of the ball from the right flank.
Purchased from Udinese last summer, the Ghanaian international has taken up the left wing-back spot to perfection.
Kwadwo Asamoah was sorely missed by Juventus during his Africa Cup tour, as none of the other replacements could offer his explosiveness on the wing.
Reborn after being deemed surplus by Milan, Andrea Pirlo is the finest deep-lying playmaker in Europe.
His resurgence ever since joining Juventus has been incredible, as he has stayed virtually injury free and was the main reason for the Bianconeri winning the Scudetto last year.
Without him, Juventus aren't the same. He's their most prized asset and the clock that makes the rest of the team tick.
The jack-of-all-trades in the midfield who can do a little bit of everything.
Bayern Munich will have to be wary of Claudio Marchisio, as his forward runs and penchant for goals are hard to defend. He's a tireless worker who can fill any role in the midfield.
Expect Claudio to play more defensively in the first leg, as he helps lead any counterattack.
Arturo Vidal is another player who knows Bayern Munich well from his days at Bayer Leverkusen, where he was noticed by Juventus.
A modern-day box-to-box midfielder, Arturo is usually plastered over the opposing playmaker.
A mercurial forward who can be world-class on his day, Mirko Vucinic is one of the most polarizing players on Juventus.
He drifts around the opposition's half, not giving defenders a reference point as he likes to slot in behind the striker or float around the wing.
Despite not being a consistent goalscorer, Mirko has a penchant for the spectacular and could be viewed as a poor man's Zlatan Ibrahimovic for his style of play.
Probably the Bianconeri's best option when they want a true target man. The ex-Milan youth product spearheads the attack and thrives on perfect service from his teammates.
While he hasn't totally convinced Juventini this season, Alessandro Matri has proven to be able to carry the load up front with a string of impressive performances in the Champions League.