Iniesta has been left off the team sheet after struggling in the first 10 minutes of practice and leaving the pitch with a calf injury.
Pep went on to say that "Iniesta is 100 percent out" and "hopefully Andres would be ready for the second leg at the Nou Camp."
Followed by that, the Blaugrana manager went on a expletive-laced tirade about how he can't compete with Jose Mourinho off the pitch, but their teams would decide what happens at the Santiago Bernabeu on Wednesday night.
This was in response to Mourinho criticizing Pep for discussing the officiating of the Copa del Rey Final that Madrid won 1-0, thanks to a Cristiano Ronaldo header in the first half of extra time.
Andres Iniesta is one half of the dynamic duo in the Barca midfield; Pep will no doubt miss the creative superstar. With Bojan Krkic also out for the remainder of the season, Adriano and Maxwell ruled out, the Catalans have a problem at left-back.
However, on a more positive note, Barca do welcome back their captain Carles Puyol and central defender Gabi Milito.
Interesting to see how this effects the tactics of each manager.
Will Pepe still play in a high defensive central midfield position? Who will tandem with Xavi Hernandez? Who will play left back for Barca? What formation will Barca play?
Injury issues offer many questions and predictions before the mouth watering tie Wednesday night in the El Clasico Champions League Semi.