After the 32-31 win, ESPN's Adam Schefter reported Barkley had a high ankle sprain and that he will undergo an MRI on Monday.
"I'm not out for the season," Barkley told reporters after the game. "I'm going to do whatever I can to get back as quickly as possible. Not for myself, but for my team. That's what I continue to think about. I caught myself getting a little upset on the sideline just because I wasn't able to be out there with those guys."
Barkley was spotted hobbling on the field with his crutches after the Giants won in dramatic fashion on a missed Matt Gay kick:
Barkley had eight carries for 10 yards and four catches for 27 yards before exiting.
The 22-year-old New York City native was selected by the Giants with the second overall pick in the 2018 NFL draft following a standout career at Penn State. As expected, he made a huge impact as a rookie, leading the league with 2,028 yards from scrimmage and scoring 15 touchdowns.
Barkley remained mostly durable throughout his time with the Nittany Lions, appearing in 38 of a possible 40 games during his three years in college. He missed time during training camp last year with a hamstring injury, but he played all 16 games during the regular season.
If the latest ailment forces him back to the sideline, Wayne Gallman should receive a majority of the touches in the New York backfield. It could also lead to some additional opportunities for fullback Elijhaa Penny in short-yardage situations.