The safety appeared to have an interception in the second quarter but the play was called back as a result of a defensive holding.
The donor said they will still give the $100,000 to PETA because the call was "100 percent unfair."
"The dogs didn't deserve that call and neither did Tyrann," they told TMZ.
The flag had a significant impact on the game as the Buccaneers scored a touchdown later in the drive on the way to a 31-9 win. However, it wasn't enough to change the planned donation.
Mathieu earned the PETA Humanitarian Award in 2020 as a result of his campaigns against dogs being left inside parked cars in the summer and outside in the winter.