MLB.com's Gregor Chisholm reported Donaldson limped off the field after suffering the injury running to first base. According to the Toronto Star's Laura Armstrong, Ryan Goins replaced him at third base.
Injuries have rarely been a problem for Donaldson, who has played in at least 155 games in each of the last four seasons.
The third baseman suffered a calf strain in the first few days of camp but otherwise hasn't missed significant time. Even when he is hurt, Donaldson doesn't allow it to affect his play.
"He's geared a certain way," manager John Gibbons said, per Jayson Stark of ESPN.com. "He feels a huge sense of obligation to his team."
This helped him miss only seven games in 2016 despite admittedly being hurt the entire year with a calf injury.
The issue also didn't stop him from finishing fourth in MVP voting thanks to his 37 home runs and 99 RBI. This was just one year after winning the MVP award in a season where he led the American League with 122 runs and 123 RBI, also registering 41 home runs and a .297 batting average.
The 31-year-old has become one of the best overall players in the majors, and his health will be vital to the Blue Jays this season.