A clash with goalkeeper Salvatore Sirigu left the 30-year-old in visible pain. After some treatment, he was unable to walk it off and was replaced by Federico Bernardeschi after just 15 minutes, per WhoScored's Ben McAleer:
The former Napoli forward moved to the Old Lady in the summer of 2016, with the Italian champions shelling out a massive £75.3 million to sign him.
Higuain's tally of 36 league goals in 35 appearances for the Partenopei in 2015-16 made him a desirable target and allowed Napoli to collect such a huge fee. He was a success in his first season in Turin, with 24 Serie A goals, and he has already bagged 14 this campaign.
The Argentina international has been in great form of late and scored twice in the UEFA Champions League match against Tottenham Hotspur, which ended 2-2. This injury ahead of the return leg on March 7 could spell disaster, adding to the club's injury issues.
Paulo Dybala has barely featured in 2018 and is slowly working his way back to full health, while Juan Cuadrado and Douglas Costa have also struggled with knocks. Most of the setbacks have come in the attacking third, where depth was already an issue.
The Bianconeri will hope their star forward can return in time for the trip to London. If not, their European adventure could come to a premature end at Wembley Stadium.