Floyd, a 46-year-old Black man, was killed after Minneapolis police officer Derek Chauvin, a white man, knelt on his neck for eight minutes and 46 seconds. Chauvin was charged with second-degree murder, third-degree murder and second-degree manslaughter, while the other three officers on the scene were charged with aiding and abetting.
Twins first-base coach Tommy Watkins took a knee during the moment of silence, according to Jim Souhan of the Star Tribune.
Twins manager Rocco Baldelli and several players kneeled during the national anthem on Opening Day as a protest against racial injustice.
"I've found that the more you look around and the more you converse with people, the more that you find a lot of common ground on a lot of different things, on a lot of different topics," Baldelli said before the game, per Betsy Helfand of the Pioneer Press. "And I find that a very beautiful thing."
The walls at Target Field also have signs that read "Justice for George Floyd" and "Black Lives Matter."