Tatis went down near third base in the bottom of the first inning after trying to advance from second on an error. He was 1-for-1 before exiting and was replaced by Ha-Seong Kim.
The MVP candidate has dealt with a shoulder issue for much of the 2021 season. He went on the injured list after being hurt in spring training and then reinjured the shoulder in June.
"I don't know how to compare them. They've all been a little bit different,'' Padres manager Jayce Tingler told reporters. "We're of the understanding that this was a possibility, the shoulder coming out and then back in. The good thing is it slid back in.
"It's so tough to predict, especially without a lot of information at this point. I will say, for whatever reason, he's had a phenomenal ability of getting back in there, honestly sooner than we've expected. But again, it's tough to say without having a lot of information.''
It's likely Tatis will be dealing with some level of shoulder discomfort throughout the season. He's managed to continue brilliant play despite being at less than 100 percent, slashing .292/.373/.651 with 31 home runs, 70 runs batted in and 23 steals.
Ha-Seong Kim will likely get work at shortstop with Tatis out of the lineup.