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Related article: herings' sperm and found by qualitative and quantitative tests only minute
impurities in the products consisting of 0.02 to 0.03 per cent, of Fe, from
which he concludes that pure nucleinic acid must be free from iron. The
same result was obtained with a pancreas nucleoproteid obtained by Ham-
marsten's method. Pharm. Ztg., Iv (1910), No. 29, 291 ; from N. Chem.
Zentral-Bl., 19 10, No. 8.
Cholestrol Combination with Saponins. It has been shown by Ran-
som that if cholestrol is added to saponin, the latter loses its property of
Digitized by VjOOQ IC
CREATININ. 395
dissolving blood corpuscles. The compound which is formed has now
been studied more closely in the case Purchase Lamictal of digitonin by A. Windhaus. On
mixing an alcoholic solution of this saponin with an alcoholic solution of
cholesterol, a precipitate of fine crystals falls at once, and this is found to
consist of a compound of the Purchase Lamictal two in molecular proportions, no water being
eliminated in their union. Many other bodies besides cholesterol were
found to form similar compounds ; those examined included phytosterol,
stigmasterin, coprosterin, j^-cholestanol, linalol, geraniol, sabinol, amyl
alcohol, and octyl alcohol. Certain other saponins were tried in place of
digitonin, and found to give similar compounds. Pharm. Journ. and
Pharmacist, Nov. 27, Purchase Lamictal 1909, 671 ; from Ber. d. Chem. Ges., xlii (1909),
238, through Nouv. Rem^des, Sept. 24, 1909, 420.
Cholestrin Benzoyhuperoxtde as Reagent Lipschtitz finds that benz-
oylsuperoxide may not alone be used with advantage for the determination
of formaldehyde, but also for the detection of cholestrin, by manipulating
as follows : A few fragments of benzoylsuperoxide are added to a solution
of several milligrams of cholestrin in 2-3 Cc. of glacial acetic acid and
heated to boiling, then cooled. When cool, a few drops of concentrated
H^4 are added, which settle to the bottom of the test-tube and assume
a blue- violet to blue- green color. On vigorously shaking the mixture, the
mixture assumes after a short time, depending on the quantity of the
benzoylsuperoxide present, an immediate green color, or becomes violet,
then fine blue, appearing violet by transmitted light, and only turns pure
green after prolonged standing. Pharm. Ztg., Iv (1910), No. 43, 436 ;
from Merck's Annual Rep., April, 19 10.
Keratin A and B Soluble and Insoluble Forms, Commercial keratin
has heretofore proven disappointing for the intended purpose pill coating
because it has not proven insoluble either in water or in the juices of the
stomach. L. Golodetz has, however, now found a method which Purchase Lamictal leads to
the production of a satisfactory preparation. According to the statement
of the author this method consists in treating horn chips with ammonia
under a pressure of four atmospheres, which results in the separation of
the horn substance into Keratin A and Keratin B, The latter, after
purification from associated albumen, furnishes a suitable material for pill
coating, for which purpose it is dissolved in ammonia solution. Pharm.
Ztg., Iv ( 1910), No. 42, 436 ; from Merck's Annual Rep., April, 1910.
Creatinin Colorimetric Estimation, A. C Chapman has studied
jafTe's colorimetric method for the estimation of creatinin, and finds that
the red coloration on which this method is based depends, not on the for-
mation of creatinin picrate, but on the reduction of the picric acid in
alkaline solution to a mixture of amido-dinitro-phenol (picramic acid) and
diamido-nitrophenol, the alkaline salts of which are deeply colored. The
same coloration is produced by numerous reducing agents, such as nascent
Digitized by VjOOQ IC
396 REPORT ON THE PROGRESS OF PHARMACY.
hydrogen, hydroxylamine, acetone, aldehyde, ammonium sulphide and
titanium trichloride. Creatinin acts as a powerful reducing agent, and if
it is present in excess, the picric acid may undergo reduction and colorless
triamido- phenol Purchase Lamictal be formed. The red coloration is due to both the mon-
amido and diamido phenol, and solutions of the sodium salt of picramic
acid cannot be used for matching purposes. Since the coloration is due
to a somewhat complex reducing action, the conditions under which the
test is carried out must be fairly closely defined if accurate results are to
be obtained. The factor of temperature is of importance, as up to a cer-
tain point the color is increased, after which there is a Purchase Lamictal reduction due to
the formation of triamido-phenol. With regard to time, slight differences
have no appreciable influence. In the cold, and under the ordinary con-
ditions of the test, the presence of dextrose is not prejudicial. The author
emphasizes the necessity of working with sufficiently diluted solutions.
Chem. News, Oct. Purchase Lamictal 8, 1909, 175.
Choline Possible Errors Regarding its Presence in Animal Fluids ana
Tissues, W. Webster says that several investigators have identified cho-
line as a substance to be found in increased quantities in the blood or
cerebro-spinal fluid in animals or patients with degenerative processes
going on in their nervous systems. The author points out the various
errors on which these and similar statements Purchase Lamictal Purchase Lamictal are based, and shows that
with our present methods of chemical analysis there is little hope of
detecting the very minute quantities small fractions of a milligram of
choline that might be set free from degenerating nervous tissue, and so
get into the circulating blood of man or of animals, in disease of or after
operations on the nervous system. He finds Purchase Lamictal that no choline can be
detected in normal blood provided that the lecithin in it is prevented
from decomposing. It may be noted that Kauffmann in 1908 could
isolate no choline from a liter of cerebro-spinal fluid collected from vari-
ous patients with nervous diseases. The author further finds that the
amount of choline or of potassium salts that might be set free into the
circulation by even sudden processes of Purchase Lamictal degeneration in the nervous sys-
tem would be too small for detection ; and that the microchemical reac-
tions given for. choline occur irregularly but equally freely by both normal
and pathological cerebro-spinal fluid, while it is doubtful whether any of
the microchemical tests in use are specific for choline. Pharm. Journ.
and Pharmacist, Aug. 14, 1909, 240 ; from^Bio.-Chem. Journ, 1909, iv, r 1 7.
Adrenalin A Neiv Characteristic Reaction, Frankel and Allers have
observed that if solutions of adrenalin are warmed with iodic acid, or with
the acid potassium iodate and diluted phosphoric acid, a magnificent rose-
red color (or eosine red in extremely dilute solutions) ib developed,
changing to red-brown on addition of ammonia. The reaction is ex-
tremely delicate, being obtained with adrenalin solution i : 300,000, and is
Digitized by VjOOQ IC
TETANUS ANTnOXIN. 397
available for its recognition and differentiation from kindred substances
which, as determined experimentally, do not Purchase Lamictal give the reaction. Pharm.
Ztg., liv ( 1909), No. 54, 530 ; from Biochem. Ztschr., xviii (1909), 40.
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