Fabrizio Giovannozzi/Associated Press
Andrea Barzagli is out with injury, so Leonardo Bonucci will be pivotal in keeping Mario Gomez quiet as he will likely be the pivot of the back three.
The German's form has suddenly resurfaced after a quiet return since his serious injury, scoring in the first leg and grabbing the match-clinching goal in the 3-1 victory over Chievo on Sunday.
Bonucci will be the deepest of the three centre-backs and will need to be alert to the former Bayern striker's clever movement, which was crucial to grabbing that away goal one week ago.
Gomez looks fit and strong, and Montella will hope that he can be alert enough inside the penalty area to make the most of his side's creativity and vision.
With Borja Valero, Alberto Aquilani and David Pizarro all likely to feature, in addition to Josip Ilicic who provided that wonderful assist for his first goal since his injury, the Viola will be confident of exposing an unsettled back line.
Juve have been troubled by the injuries to Barzagli and Giorgio Chiellini in recent weeks, in addition to this busy schedule, which has seen Conte deem it necessary to rest Bonucci as well.
This has meant that he has not been able to start all three of his Azzurri international centre-backs in one match since February 2 against Inter.
With Martin Caceres likely to complete the trio of defenders, alongside Bonucci and Chiellini, Montella must realise his best chance of upsetting Juve is to probe the organisational skills of this defence, with Gomez's movement key to finding the openings necessary to score the goals that will secure their progress to the next round.