Juve enter Sunday's big clash with Milan on the back of a superb performance in Turkey against Trabzonspor, winning 2-0, despite already leading the tie 2-0 from the first leg.
The Bianconeri may enter the clash at the San Siro with their lead at the top of Serie A cut to just three points, as Roma face Inter on Saturday.
The team was rotated somewhat on Thursday, meaning the impact of having just three days of rest before facing the Rossoneri, who did not have a midweek match, could be negated.
Paul Pogba was substituted after 37 minutes, and Andrea Pirlo made a second-half appearance as a substitute to keep his fitness ticking over.
Claudio Marchisio is getting in a rhythm too, and will be a fine replacement for the suspended Arturo Vidal.
Dani Osvaldo and Sebastian Giovinco impressed on Thursday, demonstrating great chemistry after originally playing together for the Italy under-21s.
But it will surely not be enough for Antonio Conte to consider dropping either Carlos Tevez or Fernando Llorente—at least at this stage. Should the pair continue to apply pressure by scoring goals, there may be a decision later in the season to be made.
Mauricio Isla did excellently on the right, but Stephan Lichtsteiner is one of Conte's most trusted players, and the Chilean will continue to be used sparingly whenever the Swiss international requires a rest.
Federico Peluso was less impressive and even if Kwadwo Asamoah fails to recover from the injury that kept him out of the Europa League match this week, Conte will probably opt for either Martin Caceres or Angelo Ogbonna.
Whoever does not start on the left between the Uruguayan and the Italian internationals could complete the back three with Leonardo Bonucci and the recovering Andrea Barzagli—with Giorgio Chiellini set to miss out again from a lack of match fitness after his most recent injury.
Kaka "Juve is very strong, has great talent & is doing well, so Beating Juve would give us a big charge to continue until end of the season"— Omid Zamani (@Juventus__fan) February 28, 2014
It is the third leg of a tough week for the Bianconeri, who faced Torino in the Derby della Mole, a hostile trip to Trabzon and now a dangerous league match against Milan—who may rise to the occasion, despite their league position.
The Giallorossi will be hoping that Clarence Seedorf's men can cause an upset and preserve some sort of title race, but if Juve can overcome this one, then the third successive title will almost certainly be theirs.
Expected line-up: Gianluigi Buffon; Leonardo Bonucci, Andrea Barzagli, Angelo Ogbonna; Stephan Lichtsteiner, Claudio Marchisio, Paul Pogba, Andrea Pirlo, Martin Caceres; Carlos Tevez, Fernando Llorente.