The Nottingham Boots Corsairs under-16s team made 4,489 tackles at an event on Friday beating the current record of 4,130 held by Scots College in Sydney, Australia.
The team now has to wait for final confirmation by Guinness World Records.
It must send in video evidence and reports by independent judges at the event, Rushcliffe mayor Peter McGowan and Dave Beaumont of the Notts, Leicestershire and Derbyshire Referees' Society.
The team raised about £1,000 from sponsorship for the world record bid, with players aged 15 and 16 taking part. It will be used to fund the 10-day team tour to Canada in early July.
The team has been doing car boot sales and packing bags in supermarkets over the last 18 months to raise £25,000 to go on tour.